One of the most mysterious phenomena that I have experienced through my outlook on life is the infection and outbreak of acute respiratory infections.
When I wake up every morning, the pain last night is gone, In other words, it is a so-called asymptomatic state.
During the daytime when waking up in the morning, symptoms such as burning pain in the bronchi and lungs and stomach discomfort disappear with each breath.
But, in particular, when commuting to work or by train, the pain in the throat and lungs becomes severe in the afternoon.
Perhaps there are several people who can live asymptomatic while waking up at different times depending on the person.
This cycle repeats the same every day. It’s already been over half a year.
It cannot be explained by the current human medicine and modern science.
Anyway, it is very likely that some pathogen is lurking in the central nervous system. Moreover, it uses a complex signal transduction mechanism to suppress immune function.
The proliferation cycle may depend on the intracellular activity of tissues of the nervous system somewhere, even for viral infections.
Anyway, everyone wears masks, but to tell the truth, the virus would be bypassing the masks.
It only prevents saliva from scattering due to coughing and sneezing.
However, no one notices the reality and no one knows it.
I don’t know because there is no record of the past, but since around 1990, anomalous phenomena have occurred at the world level, originating in Tokyo, Kanagawa, Shizuoka, and Aichi prefectures in Japan island.
The treatment of infected people was deemed necessary to control the global spread of the infection.
However, the infection is known to have two patterns at present.
One is asymptomatic and completely unnoticed. The other has morbid symptoms. That’s pneumonia.
I’ve noticed that there are different types of viruses that cause it.
In other words, there are apparently a wide variety of different types in the same coronavirus.
Because the reason in this point, I decided to explain in simple terms that the whole world is wrong from the beginning to the end of the PCR test protocol for the gene probe region to be tested.
Anyway, The first cause is Japan.
There is no drug that can be administered in a caliber to inhibit the growth of the virus and stop the spread of infection after infection. Apparently, intravenous antibiotics and drugs such as RNA polymerase inhibitor T-705, favipira, favilavir
, a new flu drug, and the pro-drug remdesivir,
a new nucleotide analogue pro-drug for Ebola, as well as investigational drugs in the research stage, will have to be administered intravenously.
T-705, favipira, favilavir, an antiviral drug approved in Japan as a treatment for new coronavirus COVID-19 infections,
But, remdesivir, has not been effective in improving the mortality rate of inpatients in an international clinical trial led by the World Health Organization (WHO). It was reported on October 15, 2020.
The need for a ventilator did not diminish, and there was little effect on shortening the length of hospital stay. “It seems that a pre-peer-reviewed paper using initial data has been published, which is inconsistent with the efficacy shown in another clinical trial,” said Gilead Sciences, a US pharmaceutical company that developed it.
There are several reports on similar therapeutic efficacy for the RNA polymerase inhibitor Avigan(T-705, favipira, favilavir, RNA polymerase inhibitor). In other words, depending on the medical institution, university, and research institution, the place and time are different and uncoordinated.
In conclusion, there is no effective remedy in modern science today.
What all the treatments have in common is a so-called placebo-like condition.
In addition, I believes that the pathogen of the infectious disease is not really known.
From the early stages of 2020 to the present, the only way to suppress growth is to administer intravenously with a combination of remdesivir, avigan and antibiotics.
This is the only way to control the growth from early 2020 to now.
Quite simply, there needed to be a system in which everyone with morbid symptoms went to the hospital to be tested and treated. However, the biggest problem that prevented this from happening was the isolation measures.
Isolation meant being separated from the family and social groups.
This is what makes it different from other infectious diseases such as influenza and HIV.
We realized that the cause of this is the presence of asymptomatic infected people. In other words, none of them are asymptomatic, even though they have tested positive for PCR. 99% of those tested positive were asymptomatic, an unusual phenomenon.
So now the biggest problem is that no one knows what is being tested for in detail.
Japan is trying to develop a technology to further spread the infection.
Just exhale to determine if you are infected with the new coronavirus. Such a surprising inspection method has been developed.
This is a joint research between Tohoku University and Shimadzu Corporation, a major precision equipment company, and we expect that it will be less burdensome and more advantageous than PCR testing.
On the afternoon of October 16th, President Hideo Ohno of Tohoku University and President Teruhisa Ueda of Shimadzu Corporation made a joint presentation.
The newly developed test method can determine whether or not you are infected with the new coronavirus by utilizing the latest technology that analyzes viruses and metabolites contained in your breath.
Specifically, first breathe into a dedicated device for about 5 to 10 minutes to collect the water contained in the breath. Next, viruses, proteins, metabolites, etc. are detected from the water. Detected material
By applying it to an analyzer, it can be determined whether or not it is infected with the new coronavirus after about 1 hour.
Compared to PCR tests that collect samples from the nose or mouth or use saliva as a sample, the test can be performed simply by exhaling, which is less burdensome and more accurate.
Tohoku University and Shimadzu will proceed with clinical research and put it into practical use in about a year.
I am aiming for it.
As a matter of fact, the Japanese government thinks it is unclear what it is detecting on a private-sector basis.
In other words, there is a tendency that the intelligence of Japanese people is extremely low, and everyone is confused.
Coronaviruses have a genome size of about 26 to 32 kilobases (kb), which is the largest of any RNA virus. Coronavirus RNA is the largest of the
It encodes six proteins: pp1a, pp1ab, spike glycoprotein, envelope, membrane glycoprotein, and nucleocapsidrine protein. From a study by the National Institutes of Health (NIH), the
We identified 11 regions of sequence that could predict highly pathogenic coronaviruses. The proteins encoded by these regions were pp1ab (peptides nsp3, nsp4, and nsp14), spike glycoprotein, membrane glycoprotein, and nucleocapsidrine protein. Three of these were localized in the nucleocapsid protein and one in the spike protein.
Once again, the sequence locations of the genomic regions subject to the current PCR test can be found at below urgent post.
Here, deletion: deletion (the loss of a part of the sequence) and ORF: open reading frame (the region in the sequence that is expected to be translated into a protein and working).
Economic collapse and the hardships of human society are increasing.
The cause is unknown.
Even if the well-known truth is known, it is neither a position nor a situation that can be announced.
Recently, from around June 2020, odors have been drifting from the Pacific Ocean in Japan.
It is said that it was confirmed not by one or two people but in the whole city, and reports were received at the level of tens of thousands of people. However, the cause remains unknown.
No matter how much the health bureau, the national inspection agency, and the fire department search, the place and cause of the outbreak are unknown.
However, it will not be possible to identify the court.
Therefore, it was decided to rent a measuring device from the Ministry of the Environment under the jurisdiction of the Japanese government and urgently identify the causative substance in earnest.
Some know that compounds such as pentane have been found on a regional basis. The source is still unknown.
The reason is that the time and place of receiving the report and the type of odor vary from person to person and are not constant.
Bizarre phenomena occur frequently in the island country of Japan.
It’s from 1990.
Recently, a white sphere of an unidentified projectile disappeared while moving over the Japanese archipelago at altitudes of 3000m to 5000m.
It is a fact that was taken up in the TV news because a lot of sightings and clear images were obtained with the astronomical telescope.
I don’t know what was the driving force for the flight, but it disappeared, leaving a clear image.
Japnese gavement couldn’t track where it disappeared. This is also the greatest mystery of humankind.
Currently, there are four gene probes used in the PCR test performed in the test for new type of corovirus infection.
However, in the new coronavirus, a large nucleotide sequence defect site not found in other coronaviruses is found in several gene regions.
Recently, it has also been pointed out that the onset of the disease may be related to the strength of the protein charge after the nucleus.
Perhaps other proteins also have mutations that promote proliferation.
In addition to the reality of a suicide powerhouse Japan island where the number of people who jump into trains and die every day is increasing, there are no end to the number of people who suddenly disappear from the world, in Japan.
The Ministry of Health, Labor and Welfare and the National Police Agency announced that the number of suicides in August 2020 was 1849 (national preliminary figures), an increase of 246 from the same month of the previous year. Among them, young women in their 30s or younger were particularly prominent, with a sharp increase of 74% year-on-year. For teenage girls, it has jumped 3.6 times.
Even nowadays, many women commit suicide with small suckling babies. There are many mysterious phenomena in Japan, but the reason for each is unknown.
In other words, it seems that all the minds and thoughts related to the human neocortex communicate in a state like quantum teleportation, but it cannot be explained biologically with modern science and physics.
All bizarre phenomena are the birthplace of Japan, but they are all the greatest mysteries of humankind.
According to Ministry of Internal Affairs and Communications statistics, hundreds of people evaporate and disappear every day.
I don’t know where the missing person is, but it’s listed on the Metropolitan Police Department’s database.
Also, from around June of this year, a strange odor is approaching from the Pacific Ocean. It is gradually approaching Tokyo, the capital of the inland area, from the coastline.
It is known that this phenomenon is unreasonable and disorderly, with different time zones, types of odors, and places depending on the person and without a certain order. According to the city of Yokohama, isopentane and pentane were detected in more than 10 times the amount of the detected chemical substances in the normal atmosphere as components that can be detected by modern science.
In addition, butane was detected about three times as much as usual, and ethylene and acetylene, which are raw materials for chemical products, were also detected about twice as much as usual.
However, the source of these substances is unknown.
In order to clarify this phenomenon, the Japanese government has decided to introduce and investigate equipment made with the latest Japanese technology. This is it.
Putting it all together, in short, the PCR which is doing right now in the world level, all test does not detect only SARS-CoV-2, but rather, all coronaviruses.
Probably because the current human technology cannot identify mutant strains of the same virus.
However, if many people it is quarantined including children by PCR test, it becomes a social problem. Coronaviruses are roughly classified into 7 types at the genetic level. Four of them are the causative viruses of human nasal colds. We believe that it is necessary to analyze the existing coronavirus at the genetic level in order to improve this emergency. In addition, I would like to request WHO to cooperate in securing a medical system that allows intravenous administration of therapeutic drugs only by going to the hospital without being hospitalized without isolation measures from society. At the stage of mild to moderate symptoms, the therapeutic agents are the nucleotide analog prodrug , Remdesivir.,development code GS-5734 in the United States, and the Japanese RNA polymerase inhibitor,Favipiravir(Development code T-705 , Abigan), Camostat mesilate, or Nafamostat mesylate, andIvermectin (cyclic lactone oral anthelmintic belonging to the macrolides) , which are administered intravenously in a short-term decisive battle. Once started, it may stop the growth of the virus.
If secondary infection is suspected, it is necessary to administer a combination of antibiotics. Click here to donate for this project. https://streamlabs.com/%E7%A6%BF%E5%A4%AA%E9%83%8E/tip https://streamlabs.com/禿太郎/tip
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I don’t know what the pathogen is, but I’m looking at coronaviruses that infect humans from patient tissue samples.
Therefore, coronavirus is the pathogen of acute respiratory failure.
Currently, six types of coronavirus have been known in the past. 229E and NL63 are al
Facoronavirus, HKU1, OC43, SARS-CoV, MERS-CoV are in beta
Classified into the genus Coronavirus, among these SARS-CoV and MERS-CoV
It causes severe lung disease, but the other four are only cold. Both
It has a positive-strand (messenger sense) single-strand RNA as its genome.
(On the other hand, influenza virus has a negative single-strand RNA genome.
So back to SARS-CoV-2, the genomes of the six human coronaviruses
A phylogenetic comparison with the sequence shows that SARS-CoV-2 is one of the coronaviruses.
It was found to be closely related to the genus SARS-CoV. To
However, the homology between SARS-CoV-2 and SARS-CoV is 79.6% in terms of the entire genome. Similar
They are similar, but not so similar. Binds to the receptor in the adsorbed protein
Domain (abbreviated as RBD), 6 distant amino acids in that domain
It is known that it is the point of contact with the body. Computer prediction of the three-dimensional structure of the adsorbed protein of human SARS-CoV-2 does not seem to be the optimal structure for binding. Another unique mutation has been found in the S gene of human SARS-CoV-2, which is not found in human SARS-CoV, pangolin SARS-CoV-2, or bat RaTG13. It is an amino acid sequence involved in the cleavage of adsorbed proteins. The putative cleavage site of the adsorbed protein of human SARS-CoV-2 is mutated by inserting 4 amino acids before the basic amino acid arginine (R), resulting in a PRRAR sequence. That is, proline (P) precedes, followed by 3 Rs. The insertion of P causes the protein to bend, followed by a strongly positively charged state. Next to the cleavage site, serine (S) and threonine (T) are also located, to which O-type sugar chains are bound and can be mucin (mucinous protein) -like. We have seen that the S gene of human SARS-CoV-2 has mutations in its RBD site and cleavage site.
Fig. 1 Characteristics of mutations occurring around ORF8 found in some groups of SARS-CoV-2
It is thought that the coronavirus infects humans and mutations occur repeatedly at any time in the 10,000-year history of humankind. Huge amounts of viral genome sequences are being reported by researchers around the world at an alarming rate
The reference sequence used in many documents is the 29,903-base sequence initially reported in China, which is registered with the Accession number NC_045512 in NCBI and EPI_ISL_402125 in GISAID.
Deletion (a part of the base sequence is lost)
Open reading frame (region in the base sequence that is expected to be translated into a protein and work)
(DOI: 10.1128 / mBio.01610-20 from Figure 1)
Δ382 has lost ORF8, which is a cytotoxic T cell by suppressing HLA class I (also read “The main role of HLA” below), which plays an important role in the immune system. Is not activated well, and as a result, the virus may not be properly eliminated.
Clinical data suggest that these mutations may be predominantly associated with the development of pathological symptoms according to the probability distribution, but do not seem to be the definitive cause.
Of the seven coronaviruses that infect humans, Ayal B. Gussow and colleagues at the National Institutes of Health (NIH) are highly pathogenic, SARS-CoV, SARS-CoV-2, and MERS-CoV. We conducted a study comparing the genomes of the other four coronaviruses with low levels, and found different characteristics between them. (Proceedings of the National Academy of Sciences of the United States of America) was published in the electronic version on June 10, 2020. Coronavirus RNA encodes six proteins. pp1a, pp1ab, spike glycoprotein, envelope, membrane glycoprotein, nucleocapsidrin protein. We searched for sequences that affect the difference in pathogenicity. As a result, 11 regions of the base sequence that can predict highly pathogenic coronavirus were identified. The proteins encoded by these regions were pp1ab (peptides nsp3, nsp4, nsp14), spike glycoproteins, membrane glycoproteins, and nucleocapsidrin proteins. Nucleotide sequence insertions and deletions were found in 4 of the 11 regions. Of these, three were localized to the nucleocapsid protein and one was localized to the spike protein. Insertion, deletion, and substitution of the nucleocapsid protein of the highly lethal coronavirus are caused by nuclear localization signals (two mononode-type nuclear localization signals and one binode-type nuclear localization signal) and extranuclear. It was concentrated on the export signal. Next, spike proteins and diagnostic features were examined. SARS-CoV-2 invades host cells via ACE2, and its affinity for this receptor has been reported to be 10 to 20-fold higher than SARS-CoV. The spike protein is composed of multiple domains, in which there are two heptad repeats (7-base repeat sequences), which play an essential role in infection. The fusion peptide of the spike protein is located upstream of the first heptad repeat and has an α-helical structure. The authors’ analysis found that the three highly lethal viruses inserted four amino acids in the binding region between the fusion peptide and heptad repeat1. However, this mutation was not found in the low mortality coronavirus. From these results, the authors found that the nuclear localization signal of the nucleocapsid protein was associated with the strength of coronavirus pathogenicity, and that SARS-CoV-2 and MERS-CoV had separate base sequences to the spike protein. We conclude that the insertion is involved in the infectivity of the virus and host cells to fuse. They also say that these studies may help identify coronaviruses that infect animals that are likely to infect humans in the future.
Once again, when the sequence position of the target genomic region of the current PCR test is confirmed, …
The original title is “Genomic determinants of pathogenicity in SARS-CoV-2 and other human coronaviruses”
I would like to carry out research on aging phenomena and other research support projects that suppress aging phenomena in parallel. I would like to support the treatment of aging diseases such as intractable pancreatic cancer, and to elucidate COVID-19 infectious diseases and develop preventive drugs.
It will also be used to finance the compression and solidification technology for nuclear fuel waste at nuclear power plants.